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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP32547PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti PTK6 / BRK pTyr342
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated linear peptide corresponding to Human BRK phosphorylated at Tyr342.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/mL
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Immunoblot Analysis: IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane 1) and mutant Brk(Y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(Y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(Y342).
