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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX106241 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX106241, RRID:AB_2037910
- Product name
- POLR3A antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HCT116 5% SDS PAGE GTX106241 diluted at 1:500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- POLR3A antibody detects POLR3A protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with POLR3A antibody (GTX106241) diluted at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- POLR3A antibody detects POLR3A protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with POLR3A antibody (GTX106241) diluted at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using POLR3A(GTX106241) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of POLR3A protein from SK-N-SH whole cell extracts using 5 £gg of POLR3A antibody (GTX106241).Western blot analysis was performed using POLR3A antibody (GTX106241).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
