PAB23189

antibody from Abnova Corporation

Targeting: POLR3A C160, hRPC155, RPC1, RPC155

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23189

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23189, RRID:AB_11123222

Product name

POLR3A polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant POLR3A.

Antigen sequence

PSDDNPVRANLRTKGKQYCGKGEDLCANDSYVTIQ
NSELMSGSMDKGTLGSGSKNNIFYILLRDWGQNEA
ADAMSRLARLAPVYLSNRGFSI


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with POLR3A polyclonal antibody (Cat # PAB23189) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with POLR3A polyclonal antibody (Cat # PAB23189) shows strong cytoplasmic and nuclear positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)