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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-21730 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, SK-N-AS.
- Concentration
- 0.43 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR3A using Various whole cell extracts (30 µg). Samples were loaded onto a 5% SDS-PAGE gel and probed with a POLR3A polyclonal antibody (Product # PA5-21730) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR3A using 30 µg of HCT116 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a POLR3A polyclonal antibody (Product # PA5-21730) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of POLR3A was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a POLR3A Polyclonal Antibody (Product # PA5-21730) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of POLR3A in paraformaldehyde-fixed HeLa cells using a POLR3A polyclonal antibody (Product # PA5-21730) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using POLR3A (Product # PA5-21730) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of POLR3A was performed in SK-N-SH whole cell extracts using 5 µg of POLR3A Polyclonal Antibody (Product # PA5-21730). Samples were transferred to a membrane and probed with POLR3A Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
