GTX112673

antibody from GeneTex

Targeting: MUL1 C1orf166, FLJ12875, GIDE, MAPL, MULAN, RNF218

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX112673

Provider

GeneTex

Proper citation

GeneTex Cat#GTX112673, RRID:AB_10722873

Product name

MUL1 antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: A431 10% SDS PAGE GTX112673 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

A431 whole cell and membrane extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with MUL1 antibody (GTX112673) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with MUL1 antibody (GTX112673) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with MUL1 antibody (GTX112673) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using MUL1(GTX112673) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

MUL1 antibody detects MUL1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: MUL1 stained by MUL1 antibody (GTX112673) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10£gm.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

MUL1 antibody detects MUL1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. MUL1 antibody (GTX112673) dilution: 1:500.