Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BS64014 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- MLK3 (phospho T277/S281) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human MLK3 around the phosphorylation site of Threonine 277 and Serine281.
- Description
- As a result of the binding of growth factors to their membrane receptors, cytoplasmic proteins containing Src homology 2 (SH2) domains associate with specific phosphotyrosine residues within the activated receptors and function as signaling intermediates. The action of such SH2 domain proteins frequently involves the activation of a second group of signaling intermediates characterized by SH3 domains. These latter proteins function through binding proline-rich sequences in target proteins. A novel human non-receptor protein kinase, designated either MLK3 or PTK1, is 847 amino acids in length and contains an SH3 domain in the absence of an SH2 domain. In addition, MLK3 is characterized by a leucine zipper basic region (a motif commonly associated with transcription factors) and has a long carboxy-terminal tail which exhibits proline-rich motifs similar to known SH3 binding sites. MLK3 is expressed widely and is related to the previously described MLK1 and MLK2 kinases.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of p-MLK3 (T277/S281) pAb at 1:500 dilutionLane1:HEK293Twhole cell lysateLane2:NIH-3T3 whole cell lysateLane3:PC12 whole cell lysate