Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-105817 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-MLK3 (Thr277, Ser281) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Phosphorylation of mixed lineage kinase MLK3 by cyclin-dependent kinases CDK1 and CDK2 controls ovarian cancer cell division.
Cedeno-Rosario L, Honda D, Sunderland AM, Lewandowski MD, Taylor WR, Chadee DN
The Journal of biological chemistry 2022 Aug;298(8):102263
The Journal of biological chemistry 2022 Aug;298(8):102263
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-MLK3 (Thr277, Ser281) in K562 cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-MLK3 (Thr277, Ser281) polyclonal antibody (Product # PA5-105817).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-MLK3 (Thr277, Ser281) in mouse brain. Samples were incubated with Phospho-MLK3 (Thr277, Ser281) polyclonal antibody (Product # PA5-105817).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-MLK3 (Thr277, Ser281) in human pancreas tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MLK3 (Thr277, Ser281) polyclonal antibody (Product # PA5-105817) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-MLK3 (Thr277, Ser281) in mouse lung tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MLK3 (Thr277, Ser281) polyclonal antibody (Product # PA5-105817) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.