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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-113533 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.5 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOB1 in multiple sample types using a NOB1 polyclonal antibody (Product # PA5-113533) at a dilution of 1:2,000. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: L02 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, mouse heart tissue, and mouse brain tissue.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NOB1 in multiple sample types using a NOB1 polyclonal antibody (Product # PA5-113533) at a concentration of 1.5 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Hela whole cell lysate, Mouse brain tissue, Mouse kidney tissue, Mouse stomach tissue .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemichal analysis of NOB1 in A549 cells using a Polyclonal antibody (Product # PA5-113533) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemichal analysis of NOB1 in A549 cells using a Polyclonal antibody (Product # PA5-113533) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded NOB1 in human thyroid tissue using a NOB1 Polyclonal antibody (Product # PA5-113533) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded NOB1 in human placenta tissue using a NOB1 Polyclonal antibody (Product # PA5-113533) at a dilution of 1:100.
