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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
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- Product number
- MA1-39569 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD79a Monoclonal Antibody (SP18)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with monkey, equine, bovine, porcine, rabbit, guinea pig, and chicken based on sequence homology.
- Antibody clone number
- SP18
- Concentration
- Conc. Not Determined
Submitted references Liquid-based cytologic findings of solitary extramedullary plasmacytoma in thyroid: a case report identified with fine-needle aspiration cytology.
Composite lymphoma in the anterior mediastinum: a case report and review of the literature.
Identification of rare Epstein-Barr virus infected memory B cells and plasma cells in non-monomorphic post-transplant lymphoproliferative disorders and the signature of viral signaling.
Nuclear and cytoplasmic AID in extrafollicular and germinal center B cells.
PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage.
Lee CH, Jung YY, Chung YR, Ryu HS
Diagnostic cytopathology 2014 Nov;42(11):964-9
Diagnostic cytopathology 2014 Nov;42(11):964-9
Composite lymphoma in the anterior mediastinum: a case report and review of the literature.
Yu G, Kong L, Qu G, Zhang Q, Wang W, Jiang L
Diagnostic pathology 2011 Jul 6;6:60
Diagnostic pathology 2011 Jul 6;6:60
Identification of rare Epstein-Barr virus infected memory B cells and plasma cells in non-monomorphic post-transplant lymphoproliferative disorders and the signature of viral signaling.
Shaknovich R, Basso K, Bhagat G, Mansukhani M, Hatzivassiliou G, Murty VV, Buettner M, Niedobitek G, Alobeid B, Cattoretti G
Haematologica 2006 Oct;91(10):1313-20
Haematologica 2006 Oct;91(10):1313-20
Nuclear and cytoplasmic AID in extrafollicular and germinal center B cells.
Cattoretti G, Büttner M, Shaknovich R, Kremmer E, Alobeid B, Niedobitek G
Blood 2006 May 15;107(10):3967-75
Blood 2006 May 15;107(10):3967-75
PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage.
Cattoretti G, Angelin-Duclos C, Shaknovich R, Zhou H, Wang D, Alobeid B
The Journal of pathology 2005 May;206(1):76-86
The Journal of pathology 2005 May;206(1):76-86
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-CD79a Monoclonal Antibody (SP18) (Product # MA1-39569) and a 40kDa band corresponding to CD79a was observed across cell lines tested. Whole cell extracts (30 µg lysate) of Raji (Lane 1), Ramos (Lane 2), Daudi (Lane 3), HL-60 (Lane 4), SH-SY5Y (Lane 5), NTERA-2 cl.D1 (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:5000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
