orb21585

antibody from Biorbyt

Targeting: CD79A IGA, MB-1

No application information provided.

Antibody Data

Product number

orb21585

Provider

Biorbyt

Proper citation

Biorbyt Cat#orb21585, RRID:AB_10941114

Product name

Swine IgG Against Human IgG (TRITC)

Antibody type

Polyclonal

Description

Tetramethylrhodamine isothiocyanate -conjugated IgG fraction of polyclonal swine antiserum to human IgG, Fc specific. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.

Host

Porcine

Conjugate

Orange dye

Vial size

1 ml

Concentration

IgG protein concentration 1 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.4. No foreign proteins added.

Storage

The lyophilised conjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.