A301-659A

antibody from Invitrogen Antibodies

Targeting: CEP290 3H11Ag, BBS14, CT87, FLJ13615, JBTS5, KIAA0373, LCA10, MKS4, NPHP6, POC3, rd16, SLSN6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-659A

Provider

Invitrogen Antibodies

Product name

CEP290 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CEP290 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CEP290 antibody A301-659A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CEP290 was also immunoprecipitated by rabbit anti-CEP290 antibody BL6289, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 second (B).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CEP290 by immunocytochemistry. Sample: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CEP290 (Cat. No. A301-659A) used at a dilution of 1:500 ( 2 µg/mL). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CEP290 by immunocytochemistry. Sample: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CEP290 (Cat. No. A301-659A) used at a dilution of 1:500 ( 2 µg/mL). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1:100.