Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00005004-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005004-M01, RRID:AB_546672
- Product name
- Mouse Monoclonal alpha 1-Acid Glycoprotein Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ORM1 - orosomucoid 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A serum protein profile predictive of the resistance to neoadjuvant chemotherapy in advanced breast cancers.
Hyung SW, Lee MY, Yu JH, Shin B, Jung HJ, Park JM, Han W, Lee KM, Moon HG, Zhang H, Aebersold R, Hwang D, Lee SW, Yu MH, Noh DY
Molecular & cellular proteomics : MCP 2011 Oct;10(10):M111.011023
Molecular & cellular proteomics : MCP 2011 Oct;10(10):M111.011023
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 over-expressed 293 cell line, cotransfected with ORM1 Validated Chimera RNAi ( Cat # H00005004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ORM1 monoclonal antibody (M01), clone 2F9-1F10 (Cat # H00005004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody (M01), clone 2F9-1F10.Lane 1: ORM1 transfected lysate(23.5 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - ORM1 monoclonal antibody (M01), clone 2F9-1F10 Analysis of ORM1 expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Detection limit for recombinant GST tagged ORM1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 transfected lysate using anti-ORM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ORM1 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of monoclonal antibody to ORM1 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. Antibody concentration 1 ug/ml.