MAB3694

antibody from R&D Systems

Targeting: ORM1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3694

Provider

R&D Systems

Product name

Human alpha 1-Acid Glycoprotein Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human alpha 1-Acid Glycoprotein in direct ELISAs and Western blots.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P02763


Isotype

IgG

Antibody clone number

386131

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human alpha 1-Acid Glycoprotein by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human alpha 1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for alpha 1-Acid Glycoprotein at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing and non-reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human alpha 1-Acid Glycoprotein by Western Blot. Western blot shows lysates of human plasma. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human alpha 1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha 1-Acid Glycoprotein at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

alpha 1-Acid Glycoprotein in Human Liver. alpha 1-Acid Glycoprotein was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human alpha 1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.