H00001181-M01

antibody from Abnova Corporation

Targeting: CLCN2 ClC-2, CLC2, EJM6

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001181-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001181-M01, RRID:AB_509175

Product name

CLCN2 monoclonal antibody (M01), clone 3E1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant CLCN2.

Antigen sequence

TVSTAVIVFELTGQIAHILPVMIVVILANAVAQSL
QPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDI
MVRDVPHVALSCTFRDLRLALHRTKGRMLALVESP
ESMILLGSIERSQVVALLGAQLSPARRRQHMQERR
ATQTSPLSDQEGPPTPEASVCFQVNTEDSAFPAAR
GETHKPLKPALKRGPSVTRNLGESPTGSAESAGIA
LRSLFCGSPPPEAASEKLESCEKRKLKRVRISLAS
DADLEGEMSPEETHTIFSLLGVDHAYVTSIGRLIG
IVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATS
SSDTETTEVHALWGPHSRHGLPREGSPSDSDDKCQ


Isotype

IgG

Antibody clone number

3E1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CLCN2 monoclonal antibody (M01), clone 3E1 Western Blot analysis of CLCN2 expression in Hela S3 NE ( Cat # L013V3 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CLCN2 expression in transfected 293T cell line by CLCN2 monoclonal antibody (M01), clone 3E1.Lane 1: CLCN2 transfected lysate(96.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CLCN2 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol