H00001181-M01

antibody from Invitrogen Antibodies

Targeting: CLCN2 ClC-2, CLC2, EJM6

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001181-M01

Provider

Invitrogen Antibodies

Product name

CLCN2 Monoclonal Antibody (3E1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: TVSTAVIVFE LTGQIAHILP VMIVVILANA VAQSLQPSLY DSIIRIKKLP YLPELGWGRH QQYRVRVEDI MVRDVPHVAL SCTFRDLRLA LHRTKGRMLA LVESPESMIL LGSIERSQVV ALLGAQLSPA RRRQHMQERR ATQTSPLSDQ EGPPTPEASV CFQVNTEDSA FPAARGETHK PLKPALKRGP SVTRNLGESP TGSAESAGIA LRSLFCGSPP PEAASEKLES CEKRKLKRVR ISLASDADLE GEMSPEETHT IFSLLGVDHA YVTSIGRLIG IVTLKELRKA IEGSVTAQGV KVRPPLASFR DSATSSSDTE TTEVHALWGP HSRHGLPREG SPSDSDDKCQ

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3E1

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CLCN2 expression in transfected 293T cell line by CLCN2 monoclonal antibody (M01), clone 3E1.Lane 1: CLCN2 transfected lysate(96.8 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CLCN2 over-expressed 293 cell line, cotransfected with CLCN2 Validated Chimera RNAi (Cat # H00001181-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CLCN2 monoclonal antibody (M01), clone 3E1 (Cat # H00001181-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CLCN2 expression in transfected 293T cell line by CLCN2 monoclonal antibody (M01), clone 3E1.Lane 1: CLCN2 transfected lysate(96.8 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CLCN2 monoclonal antibody (M01), clone 3E1 Western Blot analysis of CLCN2 expression in HeLa S3 NE (Cat # L013V3).

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CLCN2 is 0.3 ng/mL as a capture antibody.