H00115727-D01P

antibody from Abnova Corporation

Targeting: RASGRP4

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00115727-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00115727-D01P, RRID:AB_1712628

Product name

RASGRP4 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human RASGRP4 protein.

Antigen sequence

MNRKDSKRKSHQECTGKIGGRGRPRQVRRHKTCPS
PREISKVMASMNLGLLSEGGCSEDELLEKCIQSFD
SAGSLCHEDHMLNMVLAMHSWVLPSADLAARLLTS
YQKATGDTQELRRLQICHLVRYWLMRHPEVMHQDP
QLEEVIGRFWATVAREGNSAQRRLGDSSDLLSPGG
PGPPLPMSSPGLGKKRKVSLLFDHLETGELAQHLT
YLEFRSFQAITPQDLRSYVLQGSVRGCPALEGSVG
LSNSVSRWVQVMVLSRPGPLQRAQVLDKFIHVAQR
LHQLQNFNTLMAVTGGLCHSAISRLKDSHAHLSPD
STKALLELTELLASHNNYARYRRTWAGCAGFRLPV
LGVHLKDLVSLHEAQPDRLPDGRLHLPKLNNLYLR
LQELVALQGQHPPCSANEDLLHLLTLSLDLFYTED
EIYELSYAREPRCPKSLPPSPFNAPLVVEWAPGVT
PKPDRVTLGRHVEQLVESVFKNYDPEGRGTISQED
FERLSGNFPFACHGLHPPPRQGRGSFSREELTGYL
LRASAICSKLGLAFLHTFHEVTFRKPTFCDSCSGF
LWGVTKQGYRCRECGLCCHKHCRDQVKVECKKRPG
AKGDAGPPGAPVPSTPAPHASCGSEENHSYTLSLE
PETGCQLRHAWTQTESPHPSWETDTVPCPVMDPPS
TASSKLDS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RASGRP4 expression in transfected 293T cell line (H00115727-T01) by RASGRP4 MaxPab polyclonal antibody.Lane 1: RASGRP4 transfected lysate(74.90 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)