Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN951666 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Cornichon Homolog 2 (Drosophila) (CNIH2) (N-Term), (AA 38-67) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 38-67 amino acids from the N-terminal region of human CNIH2
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 38-67
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- CNIH2 Antibody (N-term) (Cat. #AP50986PU-N) western blot analysis in HL-60 cell line lysates (35μg/lane).This demonstrates the CNIH2 antibody detected the CNIH2 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of CNIH2 Antibody (N-term)(Cat#AP50986PU-N) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- CNIH2 Antibody (N-term) (Cat. #AP50986PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CNIH2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- CNIH2 Antibody (N-term) (Cat. #AP50986PU-N) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.