ABIN955327
antibody from antibodies-online
Targeting: TRERF1
BCAR2, dJ139D8.5, HSA277276, RAPA, TReP-132
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN955327 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Transcriptional Regulating Factor 1 (TRERF1) (C-Term), (AA 1143-1172) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 1143~1172 amino acids from the C-terminal region of human TREF1
- Description
- Protein A column followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 1143-1172
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store at 2 - 8°C for up to six months or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references TReP-132 is a novel progesterone receptor coactivator required for the inhibition of breast cancer cell growth and enhancement of differentiation by progesterone.
TReP-132 controls cell proliferation by regulating the expression of the cyclin-dependent kinase inhibitors p21WAF1/Cip1 and p27Kip1.
Large-scale characterization of HeLa cell nuclear phosphoproteins.
The transcriptional regulating protein of 132 kDa (TReP-132) differentially influences steroidogenic pathways in human adrenal NCI-H295 cells.
Gizard F, Robillard R, Gross B, Barbier O, Révillion F, Peyrat JP, Torpier G, Hum DW, Staels B
Molecular and cellular biology 2006 Oct;26(20):7632-44
Molecular and cellular biology 2006 Oct;26(20):7632-44
TReP-132 controls cell proliferation by regulating the expression of the cyclin-dependent kinase inhibitors p21WAF1/Cip1 and p27Kip1.
Gizard F, Robillard R, Barbier O, Quatannens B, Faucompré A, Révillion F, Peyrat JP, Staels B, Hum DW
Molecular and cellular biology 2005 Jun;25(11):4335-48
Molecular and cellular biology 2005 Jun;25(11):4335-48
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
The transcriptional regulating protein of 132 kDa (TReP-132) differentially influences steroidogenic pathways in human adrenal NCI-H295 cells.
Gizard F, Teissier E, Dufort I, Luc G, Luu-The V, Staels B, Hum DW
Journal of molecular endocrinology 2004 Apr;32(2):557-69
Journal of molecular endocrinology 2004 Apr;32(2):557-69
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of TREF1 Antibody (C-term) (AP54355PU-N) in K562 cell line lysates (35 μg/lane). TREF1 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- TREF1 Antibody (C-term) (AP54355PU-N) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.