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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- PPS074 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal IFN-alpha/beta R1 Antibody
- Antibody type
- Polyclonal
- Description
- IFN-alpha/beta R1
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage, = -20degrees C is recommended. Product is stable at = -20degrees C for at least 1 year.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Phospho-IFN-alpha/beta R1 (S535/S539) by Western Blot Western blot of immunoprecipitates from HEK293 cells transfected with 1) Mock, 2) IFN-alpha R1 wild type, and 3) IFN-alpha R1 S535A and S539A mutants showing specific immunolabeling of the ~110 kDa to ~130 kDa IFN-alpha R1 wild type. The immunolabeling is absent in the IFN-alpha R1 S535 and S539 mutants (Control). The immunolableing is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).
