ARG52325

antibody from Arigo

Targeting: IFNAR1 IFNAR, IFRC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ARG52325

Provider

Arigo

Product name

anti-IFNAR1 phospho (Ser535 / Ser539) antibody

Antibody type

Polyclonal

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 conjugated to KLH

Description

Affinity Purified

Reactivity

Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Storage

Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western Blot: immunoprecipitates from HEK 293 cells transfected with 1. Mock 2. IFNAR1 WT and 3. IFNAR1 Ser 535 Arg and Ser 539 Arg mutants stained with ARG52325 anti-IFNAR1 (phospho Ser535 / Ser539) antibody (ARG52325)The immunolabeling is absent in the IFNAR1 Ser 535 and Ser 539 mutants. The labeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the dephosphopeptide (Dephos). immunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT, and 3. IFNAR1 S535A and S539A mutants showing specific immunolabeling of the ~110k to ~130k IFNAR1 WT. The immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (Control). The immunolabeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).