Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- PAB9607 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9607, RRID:AB_1708362
- Product name
- IFNAR1 (phospho S535/S539) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of IFNAR1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references SCF(HOS) ubiquitin ligase mediates the ligand-induced down-regulation of the interferon-alpha receptor.
Cancer immunotherapy: the interferon-alpha experience.
Interferons alpha and beta as immune regulators--a new look.
Kumar KG, Tang W, Ravindranath AK, Clark WA, Croze E, Fuchs SY
The EMBO journal 2003 Oct 15;22(20):5480-90
The EMBO journal 2003 Oct 15;22(20):5480-90
Cancer immunotherapy: the interferon-alpha experience.
Kirkwood J
Seminars in oncology 2002 Jun;29(3 Suppl 7):18-26
Seminars in oncology 2002 Jun;29(3 Suppl 7):18-26
Interferons alpha and beta as immune regulators--a new look.
Biron CA
Immunity 2001 Jun;14(6):661-4
Immunity 2001 Jun;14(6):661-4
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot of immunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT, and 3. IFNAR1 S535A and S539A mutants showing specific immunolabeling of the ~110k to ~130k IFNAR1 WT. The immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (Control). The immunolabeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).
- Validation comment
- Immunoprecipitation