PPS074

antibody from R&D Systems

Targeting: IFNAR1 IFNAR, IFRC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PPS074

Provider

R&D Systems

Product name

Human Phospho-IFN-alpha/beta R1 (S535/S539) Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Human IFN-alpha R1, Type I, Subunit I phosphorylated at S535/S539

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 uL

Storage

For long-term storage,

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Phospho-IFN-alpha/beta R1 (S535/S539) by Western Blot Western blot of immunoprecipitates from HEK293 cells transfected with 1) Mock, 2) IFN-alpha R1 wild type, and 3) IFN-alpha R1 S535A and S539A mutants showing specific immunolabeling of the ~110 kDa to ~130 kDa IFN-alpha R1 wild type. The immunolabeling is absent in the IFN-alpha R1 S535 and S539 mutants (Control). The immunolableing is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).