Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 15917-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TBCC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MESVSCSAA AVRTGDMESQ RDLSLVPERL QRREQERQLE VERRKQKRQN QEVEKENSHF FVATFARERA AVEELLERAE SVERLEEAAS RLQGLQKLIN DSVFFLAAYD LRQGQEALAR LQAALAERRR GLQPKKRFAF KTRGKDAASS TKVDAAPGIP PAVESIQDSP LPKKAEGDLG PSWVCGFSNL ESQVLEKRAS ELHQRDVLLT ELSNCTVRLY GNPNTLRLTK AHSCKLLCGP VSTSVFLEDC SDCVLAVACQ QLRIHSTKDT RIFLQVTSRA IVEDCSGIQF APYTWSYPEI DKDFESSGLD RSKNNWNDVD DFNWLARDMA SPNWSILPEE ERNIQWD (1-346 aa encoded by BC020170)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat liver tissue were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using TBCC antibody 15917-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug TBCC antibody (15917-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.