Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-90023 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFE2L1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SSSFSEEGAV GYSSDSETLD LEEAEGAVGY QPEYSKFCRM SYQDPAQLSC LPYLEHVGHN HTYNMAPSAL DSADLPPPSA LKKGSKEKQA DFLDKQMSRD EHRARAMKIP FTNDKIINLP VEEFNELLSK YQLSEAQLSL IRDIRRRGKN KMAAQNCRKR KLDTILNLER DVEDLQRDKA RLLREKVEFL RSLRQMKQKV QSLYQEVFGR LRDENGRPYS PSQYALQYAG DGSVLLIPRT MADQQARRQE RKPKDRRK; Positive Samples: HepG2, Jurkat, PC-12, Mouse fat, Mouse lung, Mouse liver; Cellular Location: Endoplasmic reticulum membrane, Nucleus, Single-pass type II membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.51 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using NFE2L1 Polyclonal antibody (Product # PA5-90023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFE2L1 in extracts of various cell lines using NFE2L1 Polyclonal Antibody (Product # PA5-90023) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NFE2L1 was performed in HeLa cells using NFE2L1 Polyclonal Antibody (Product # PA5-90023).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFE2L1 in paraffin-embedded rat spleen using NFE2L1 Polyclonal Antibody (Product # PA5-90023) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFE2L1 in paraffin-embedded rat heart using NFE2L1 Polyclonal Antibody (Product # PA5-90023) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFE2L1 in paraffin-embedded human lung cancer using NFE2L1 Polyclonal Antibody (Product # PA5-90023) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFE2L1 in paraffin-embedded human colon using NFE2L1 Polyclonal Antibody (Product # PA5-90023) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFE2L1 in paraffin-embedded human placenta using NFE2L1 Polyclonal Antibody (Product # PA5-90023) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFE2L1 in paraffin-embedded human gastric cancer using NFE2L1 Polyclonal Antibody (Product # PA5-90023) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFE2L1 in paraffin-embedded human uterus using NFE2L1 Polyclonal Antibody (Product # PA5-90023) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFE2L1 in paraffin-embedded mouse brain using NFE2L1 Polyclonal Antibody (Product # PA5-90023) at a dilution of 1:100.
