LS-B12944

antibody from LSBio

Targeting: LIPA CESD, LAL

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B12944

Provider

LSBio

Product name

IHC-plus™ LIPA / Lysosomal Acid Lipase Antibody (clone 1F9) LS-B12944

Antibody type

Monoclonal

Description

Purified from ascites by Protein A

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1F9

Storage

Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody (M01), clone 1F9.Lane 1: LIPA transfected lysate(45 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection limit for recombinant GST tagged LIPA is approximately 10 ng/ml as a capture antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged LIPA is approximately 10 ng/ml as a capture antibody.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPA rabbit polyclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPA rabbit polyclonal antibody.