Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-82508 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TOM70 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PLLSTQDFNM AADIDPQNAD VYHHRGQLKI LLDQVEEAVA DFDECIRLRP ESALAQAQKC FALYRQAYTG NNSSQIQAAM KGFEEVIKKF PRCAEGYALY AQALTDQQQF GKADEMYDKC IDLEPDNATT
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Pathologically phosphorylated tau at S396/404 (PHF-1) is accumulated inside of hippocampal synaptic mitochondria of aged Wild-type mice.
Torres AK, Jara C, Olesen MA, Tapia-Rojas C
Scientific reports 2021 Feb 24;11(1):4448
Scientific reports 2021 Feb 24;11(1):4448
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TOM70 by a TOM70 polyclonal antibody (Product # PA5-82508). Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TOM70 by a TOM70 polyclonal antibody (Product # PA5-82508). Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TOM70 in A-431 cells using a TOM70 polyclonal antibody (Product # PA5-82508). The analysis shows localization to mitochondria.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TOM70 in human cerebral cortex and pancreas tissues using a TOM70 polyclonal antibody (Product # PA5-82508). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TOM70 in human cerebral cortex using a TOM70 polyclonal antibody (Product # PA5-82508). The analysis shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TOM70 in human pancreas using a TOM70 polyclonal antibody (Product # PA5-82508). The analysis shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Phosphorylated tau at Ser396/404 (PHF-1) epitopes increased during aging in the hippocampus. ( a ) Schematic representation of tau protein and the phosphorylation at Ser404/396 residues, epitope known as PHF-1. Created with BioRender.com, ( b ) Western blot of tau PHF-1 in hippocampal tissue of 3 and 18-month-old (mo) mice. Densitometric analysis is shown as relative values to the control. ( c ) Representative immunofluorescence images for PHF-1 tau DG, CA1, and CA3 hippocampal regions, with its quantitative fluorescence analysis. ( d ). Double staining for Tom70 (mitochondrial marker; green) and PHF-1 (Red) to analyze the colocalization of phosphorylated tau with mitochondria, in DG, CA1 and CA3 hippocampal regions. White arrows show co-localization between PHF-1 tau and mitochondria.The images were taken using a ( c ) 20 x objective and ( d ) 63 x objective. n = 3 different animals of each age. Graph bars represent means +- SEM. * p < 0.05. ** p < 0.01; *** p < 0.001. DG Dentate Gyrus, CA1 Cornu ammonis 1 and CA3 Cornu Ammonis 3.