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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- TA500948 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500948, RRID:AB_11125836
- Product name
- IFIT1 mouse monoclonal antibody, clone OTI3G8 (formerly 3G8)
- Antibody type
- Monoclonal
- Description
- IFIT1 mouse monoclonal antibody, clone OTI3G8 (formerly 3G8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- IFIT1
- Isotype
- IgG
- Antibody clone number
- OTI3G8
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references IFIT1 Expression Patterns Induced by H9N2 Virus and Inactivated Viral Particle in Human Umbilical Vein Endothelial Cells and Bronchus Epithelial Cells.
Feng B, Zhang Q, Wang J, Dong H, Mu X, Hu G, Zhang T
Molecules and cells 2018 Apr 30;41(4):271-281
Molecules and cells 2018 Apr 30;41(4):271-281
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIT1 (RC203320, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIT1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-IFIT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-IFIT1 mouse monoclonal antibody (TA500948).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-IFIT1 mouse monoclonal antibody (TA500948) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT1(RC203320).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IFIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500948)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-IFIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500948)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IFIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500948)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-IFIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500948)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC203320 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IFIT1 antibody(TA500948), and then analyzed by flow cytometry.
- Validation comment
- FC
