Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 21324-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZDHHC5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: AMPHSSSAK LSRGDSLKEP TSIAESSRHP SYRSEPSLEP ESFRSPTFGK SFHFDPLSSG SRSSSLKSAQ GTGFELGQLQ SIRSEGTTST SYKSLANQTR NGSLSYDSLL TPSDSPDFES VQAGPEPDPP LGYTSPFLSA RLAQQREAER HPRLVPTGPT HREPSPVRYD NLSRHIVASL QEREKLLRQS PPLPGREEEP GLGDSGIQST PGSGHAPRTS SSSDDSKRSP LGKTPLGRPA VPRFGKPDGL RGRGVGSPEP GPTAPYLGRS MSYSSQKAQP GVSETEEVAL QPLLTPKDEV QLKTTYSKSN GQPKSLGSAS PGPGQPPLSS PTRGGVKKVS GVGGTTYEIS V (366-715 aa encoded by BC026967)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using ZDHHC5 antibody 21324-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug ZDHHC5 antibody (21324-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IGG with dilution 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ZDHHC5 (IP:21324-1-AP, 4ug; Detection:21324-1-AP 1:500) with HEK-293 cells lysate 1680 ug.