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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB5445 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal SALM3/LRFN4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human SALM3/LRFN4 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) SALM1, approximately 20-25% cross-reactivity with recombinant human rhSALM2, and no cross-reactivity with rhSALM4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references High SALM3 Expression in Tumor Cells and Fibroblasts Is Correlated with Poor Prognosis in Gastric Cancer Patients.
Liu Y, Chen X, Chen X, Yang X, Song Q, Wu H
Disease markers 2019;2019:8282414
Disease markers 2019;2019:8282414
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- SALM3/LRFN4 in Human Brain. SALM3/LRFN4 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human SALM3/LRFN4 Monoclonal Antibody (Catalog # MAB5445) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
