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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF1598 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-29/IFN-lambda 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-29/IFN-lambda 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) IL-28A and rhIL-28B is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The Effect of Chronic Hepatitis B Virus Infection on BDCA3+ Dendritic Cell Frequency and Function.
Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans.
Adenoviruses-mediated transduction of human oesophageal carcinoma cells with the interferon-λ genes produced anti-tumour effects.
Respiratory virus induction of alpha-, beta- and lambda-interferons in bronchial epithelial cells and peripheral blood mononuclear cells.
Human TLR-7-, -8-, and -9-mediated induction of IFN-alpha/beta and -lambda Is IRAK-4 dependent and redundant for protective immunity to viruses.
van der Aa E, Buschow SI, Biesta PJ, Janssen HL, Woltman AM
PloS one 2016;11(8):e0161235
PloS one 2016;11(8):e0161235
Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans.
Popugaeva E, Witkowski PT, Schlegel M, Ulrich RG, Auste B, Rang A, Krüger DH, Klempa B
PloS one 2012;7(4):e35587
PloS one 2012;7(4):e35587
Adenoviruses-mediated transduction of human oesophageal carcinoma cells with the interferon-λ genes produced anti-tumour effects.
Li Q, Kawamura K, Okamoto S, Fujie H, Numasaki M, Namba M, Nagata M, Shimada H, Kobayashi H, Tagawa M
British journal of cancer 2011 Oct 25;105(9):1302-12
British journal of cancer 2011 Oct 25;105(9):1302-12
Respiratory virus induction of alpha-, beta- and lambda-interferons in bronchial epithelial cells and peripheral blood mononuclear cells.
Khaitov MR, Laza-Stanca V, Edwards MR, Walton RP, Rohde G, Contoli M, Papi A, Stanciu LA, Kotenko SV, Johnston SL
Allergy 2009 Mar;64(3):375-86
Allergy 2009 Mar;64(3):375-86
Human TLR-7-, -8-, and -9-mediated induction of IFN-alpha/beta and -lambda Is IRAK-4 dependent and redundant for protective immunity to viruses.
Yang K, Puel A, Zhang S, Eidenschenk C, Ku CL, Casrouge A, Picard C, von Bernuth H, Senechal B, Plancoulaine S, Al-Hajjar S, Al-Ghonaium A, Maródi L, Davidson D, Speert D, Roifman C, Garty BZ, Ozinsky A, Barrat FJ, Coffman RL, Miller RL, Li X, Lebon P, Rodriguez-Gallego C, Chapel H, Geissmann F, Jouanguy E, Casanova JL
Immunity 2005 Nov;23(5):465-78
Immunity 2005 Nov;23(5):465-78
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- IL-29/IFN-lambda 1 Inhibition of EMCV-induced Cytopathy and Neutralization by Human IL-29/IFN-lambda 1 Antibody. Recombinant Human IL-29/ IFN-lambda 1 (Catalog # 1598-IL) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepato-cellular carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IL-29/IFN-lambda 1 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-29/IFN-lambda 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1598). The ND50 is typically 0.2-1.0 µg/mL.
