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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP2-36523 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Poly-Ubiquitin Antibody
- Antibody type
- Monoclonal
- Description
- Ion exchange chromatography. Antibody specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitin specific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis of HeLa S100 Fraction. (Lane A) and MAb to Polyubiquitinylated Conjugates (clone FK1) (Lane B) at 1:1000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis at 1:1000 dilution
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Sections of hippocampus from human Alzheimers disease brain stained at 1:100 dilution with biotinylated secondary antibody and DAB detection.
