Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
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Validation data
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- Product number
- MAB8595 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Ubiquitin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human Ubiquitin in direct ELISAs
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P0CG47
- Isotype
- IgG
- Antibody clone number
- 1002A
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Ubiquitin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated with MG132. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Ubiquitin at various molecular weights (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Ubiquitin by Western Blot. Western blot shows Di-Ubiquitin chains of the indicated linkage type loaded at 2 ng/lane. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Di-Ubiquitin as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.