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- Antibody Data
- Antigen structure
- References [25]
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- Western blot [3]
- Immunohistochemistry [4]
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- Product number
- NB300-130 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-130, RRID:AB_2238516
- Product name
- Mouse Monoclonal Ubiquitin Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified. Recognizes polyubiquitin chains much more strongly than monoubiquitinated molecules or free ubiquitin. Specifically recognizes ubiquitinated cytoplasmic inclusion bodies.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Drosophila, Porcine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Melatonin-stimulated exosomes enhance the regenerative potential of chronic kidney disease-derived mesenchymal stem/stromal cells via cellular prion proteins.
New insights into intranuclear inclusions in thyroid carcinoma: Association with autophagy and with BRAFV600E mutation.
M1-linked ubiquitination by LUBEL is required for inflammatory responses to oral infection in Drosophila.
Small molecule modulator of aggrephagy regulates neuroinflammation to curb pathogenesis of neurodegeneration.
Insights into ubiquitin chain architecture using Ub-clipping.
Salmonella escapes antigen presentation through K63 ubiquitination mediated endosomal proteolysis of MHC II via modulation of endosomal acidification in dendritic cells.
Mechanism of parkin activation by PINK1.
Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked Ubiquitin Signaling.
Mechanism and regulation of the Lys6-selective deubiquitinase USP30.
Systemic inflammation is associated with exaggerated skeletal muscle protein catabolism in maintenance hemodialysis patients.
Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins.
The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases.
The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.
Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation.
Docosahexaenoic acid reduces ER stress and abnormal protein accumulation and improves neuronal function following traumatic brain injury.
Tubulin polymerization-promoting protein (TPPP/p25α) promotes unconventional secretion of α-synuclein through exophagy by impairing autophagosome-lysosome fusion.
Requirement of PML SUMO interacting motif for RNF4- or arsenic trioxide-induced degradation of nuclear PML isoforms.
Analysis of macroautophagy by immunohistochemistry.
COP9 signalosome- and 26S proteasome-dependent regulation of SCFTIR1 accumulation in Arabidopsis.
Suppression of the deubiquitinating enzyme USP5 causes the accumulation of unanchored polyubiquitin and the activation of p53.
A drosophila model for amyotrophic lateral sclerosis reveals motor neuron damage by human SOD1.
The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover.
Alzheimer disease-specific conformation of hyperphosphorylated paired helical filament-Tau is polyubiquitinated through Lys-48, Lys-11, and Lys-6 ubiquitin conjugation.
Identification of metabolic enzymes in renal cell carcinoma utilizing PROTEOMEX analyses.
Identification of metabolic enzymes in renal cell carcinoma utilizing PROTEOMEX analyses.
Yoon YM, Lee JH, Song KH, Noh H, Lee SH
Journal of pineal research 2020 Apr;68(3):e12632
Journal of pineal research 2020 Apr;68(3):e12632
New insights into intranuclear inclusions in thyroid carcinoma: Association with autophagy and with BRAFV600E mutation.
Schwertheim S, Theurer S, Jastrow H, Herold T, Ting S, Westerwick D, Bertram S, Schaefer CM, Kälsch J, Baba HA, Schmid KW
PloS one 2019;14(12):e0226199
PloS one 2019;14(12):e0226199
M1-linked ubiquitination by LUBEL is required for inflammatory responses to oral infection in Drosophila.
Aalto AL, Mohan AK, Schwintzer L, Kupka S, Kietz C, Walczak H, Broemer M, Meinander A
Cell death and differentiation 2019 May;26(5):860-876
Cell death and differentiation 2019 May;26(5):860-876
Small molecule modulator of aggrephagy regulates neuroinflammation to curb pathogenesis of neurodegeneration.
Sn S, Pandurangi J, Murumalla R, Dj V, Garimella L, Acharya A, Rai S, Paul A, Yarreiphang H, Pillai MS, Giridharan M, Clement JP, Alladi PA, Saiyed T, Manjithaya R
EBioMedicine 2019 Dec;50:260-273
EBioMedicine 2019 Dec;50:260-273
Insights into ubiquitin chain architecture using Ub-clipping.
Swatek KN, Usher JL, Kueck AF, Gladkova C, Mevissen TET, Pruneda JN, Skern T, Komander D
Nature 2019 Aug;572(7770):533-537
Nature 2019 Aug;572(7770):533-537
Salmonella escapes antigen presentation through K63 ubiquitination mediated endosomal proteolysis of MHC II via modulation of endosomal acidification in dendritic cells.
Gogoi M, Ravikumar V, Dixit NM, Chakravortty D
Pathogens and disease 2018 Mar 1;76(2)
Pathogens and disease 2018 Mar 1;76(2)
Mechanism of parkin activation by PINK1.
Gladkova C, Maslen SL, Skehel JM, Komander D
Nature 2018 Jul;559(7714):410-414
Nature 2018 Jul;559(7714):410-414
Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked Ubiquitin Signaling.
Michel MA, Swatek KN, Hospenthal MK, Komander D
Molecular cell 2017 Oct 5;68(1):233-246.e5
Molecular cell 2017 Oct 5;68(1):233-246.e5
Mechanism and regulation of the Lys6-selective deubiquitinase USP30.
Gersch M, Gladkova C, Schubert AF, Michel MA, Maslen S, Komander D
Nature structural & molecular biology 2017 Nov;24(11):920-930
Nature structural & molecular biology 2017 Nov;24(11):920-930
Systemic inflammation is associated with exaggerated skeletal muscle protein catabolism in maintenance hemodialysis patients.
Deger SM, Hung AM, Gamboa JL, Siew ED, Ellis CD, Booker C, Sha F, Li H, Bian A, Stewart TG, Zent R, Mitch WE, Abumrad NN, Ikizler TA
JCI insight 2017 Nov 16;2(22)
JCI insight 2017 Nov 16;2(22)
Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins.
Kaul Z, Chakrabarti O
Molecular biology of the cell 2017 Jul 15;28(15):2106-2122
Molecular biology of the cell 2017 Jul 15;28(15):2106-2122
The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases.
Pruneda JN, Durkin CH, Geurink PP, Ovaa H, Santhanam B, Holden DW, Komander D
Molecular cell 2016 Jul 21;63(2):261-276
Molecular cell 2016 Jul 21;63(2):261-276
The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.
Damgaard RB, Walker JA, Marco-Casanova P, Morgan NV, Titheradge HL, Elliott PR, McHale D, Maher ER, McKenzie ANJ, Komander D
Cell 2016 Aug 25;166(5):1215-1230.e20
Cell 2016 Aug 25;166(5):1215-1230.e20
Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation.
Schwab AJ, Ebert AD
Stem cell reports 2015 Dec 8;5(6):1039-1052
Stem cell reports 2015 Dec 8;5(6):1039-1052
Docosahexaenoic acid reduces ER stress and abnormal protein accumulation and improves neuronal function following traumatic brain injury.
Begum G, Yan HQ, Li L, Singh A, Dixon CE, Sun D
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Mar 5;34(10):3743-55
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Mar 5;34(10):3743-55
Tubulin polymerization-promoting protein (TPPP/p25α) promotes unconventional secretion of α-synuclein through exophagy by impairing autophagosome-lysosome fusion.
Ejlerskov P, Rasmussen I, Nielsen TT, Bergström AL, Tohyama Y, Jensen PH, Vilhardt F
The Journal of biological chemistry 2013 Jun 14;288(24):17313-35
The Journal of biological chemistry 2013 Jun 14;288(24):17313-35
Requirement of PML SUMO interacting motif for RNF4- or arsenic trioxide-induced degradation of nuclear PML isoforms.
Maroui MA, Kheddache-Atmane S, El Asmi F, Dianoux L, Aubry M, Chelbi-Alix MK
PloS one 2012;7(9):e44949
PloS one 2012;7(9):e44949
Analysis of macroautophagy by immunohistochemistry.
Rosenfeldt MT, Nixon C, Liu E, Mah LY, Ryan KM
Autophagy 2012 Jun;8(6):963-9
Autophagy 2012 Jun;8(6):963-9
COP9 signalosome- and 26S proteasome-dependent regulation of SCFTIR1 accumulation in Arabidopsis.
Stuttmann J, Lechner E, Guérois R, Parker JE, Nussaume L, Genschik P, Noël LD
The Journal of biological chemistry 2009 Mar 20;284(12):7920-30
The Journal of biological chemistry 2009 Mar 20;284(12):7920-30
Suppression of the deubiquitinating enzyme USP5 causes the accumulation of unanchored polyubiquitin and the activation of p53.
Dayal S, Sparks A, Jacob J, Allende-Vega N, Lane DP, Saville MK
The Journal of biological chemistry 2009 Feb 20;284(8):5030-41
The Journal of biological chemistry 2009 Feb 20;284(8):5030-41
A drosophila model for amyotrophic lateral sclerosis reveals motor neuron damage by human SOD1.
Watson MR, Lagow RD, Xu K, Zhang B, Bonini NM
The Journal of biological chemistry 2008 Sep 5;283(36):24972-81
The Journal of biological chemistry 2008 Sep 5;283(36):24972-81
The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover.
Chang L, Kamata H, Solinas G, Luo JL, Maeda S, Venuprasad K, Liu YC, Karin M
Cell 2006 Feb 10;124(3):601-13
Cell 2006 Feb 10;124(3):601-13
Alzheimer disease-specific conformation of hyperphosphorylated paired helical filament-Tau is polyubiquitinated through Lys-48, Lys-11, and Lys-6 ubiquitin conjugation.
Cripps D, Thomas SN, Jeng Y, Yang F, Davies P, Yang AJ
The Journal of biological chemistry 2006 Apr 21;281(16):10825-38
The Journal of biological chemistry 2006 Apr 21;281(16):10825-38
Identification of metabolic enzymes in renal cell carcinoma utilizing PROTEOMEX analyses.
Lichtenfels R, Kellner R, Atkins D, Bukur J, Ackermann A, Beck J, Brenner W, Melchior S, Seliger B
Biochimica et biophysica acta 2003 Mar 21;1646(1-2):21-31
Biochimica et biophysica acta 2003 Mar 21;1646(1-2):21-31
Identification of metabolic enzymes in renal cell carcinoma utilizing PROTEOMEX analyses.
Lichtenfels R, Kellner R, Atkins D, Bukur J, Ackermann A, Beck J, Brenner W, Melchior S, Seliger B
Biochimica et biophysica acta 2003 Mar 21;1646(1-2):21-31
Biochimica et biophysica acta 2003 Mar 21;1646(1-2):21-31
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Western blot of Ubiquitin expression on (Lane 1) poly-ubiquitin (lys63 linked), (Lane 2) pure ubiquitin and crude homogenates of adult rat, (Lane 3) cortex, (Lane 4) cerebellum and (Lane 5) brain stem.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Analysis of HEK293 cell lysates using mouse mAb Ubiquitin, dilution 1:1000 (Green). [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with 10uM of proteasome inhibitor lactacystin (Lc) for 16hrs. Lysed cells were electrophoresed on 4-20% SDS-PAGE and transferred to PVDF membranes. The smear detected above the 200kDa standard represent accumulation of ubiquitinated proteins in proteasome inhibitor-Lc treated cells. The prominent band at 8kDa corresponds to monoubiquitin. Rabbit pAb to HSP60, dilution 1:5000 (Red) was used as a loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Ubiquitination of PML in response to As2O3. PML-/- MEFs were transiently transfected with the empty vector (EV) or PMLIII (left panel) or contructs encoding PMLIII, PMLIII-SIM or PMLVI (right panel). Two days later, they were treated with As2O3 for 1 h. The cell extracts were immunoprecipitated with rabbit anti-PML antibody and the immunoprecipitate was analyzed by Western blot using anti-ubiquitin and anti-PML antibodies. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044949) licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - IHC staining of Ubiquitin in human rectal cancer using DAB with hematoxylin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - Staining of Ubiquitin in hippocampal tissue from an Alzheimer patient.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - IHC-P staining of cerebral cortex tissue from an Alzheimer patient brain using Ubiquitin antibody (clone Ubi-1) at 1:1000 dilution. Neurofibrillary tangles and dystrophic neurites associated with senile plaques stain strongly with this antibody. In the center is a typical neurofibrillary tangle containing neuron.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - FFPE section of cerebral cortex of an Alzheimer patient processed with Ubiquitin antibody using HRP/DAB (Brown) and also stained with haemotoxylin (Blue). A typical flame shaped tangle is seen in a pyramidal neuron in the center and is surrounded by some dystrophic neurites, also strongly ubiquitin positive. Both are commonly seen in cortical and hippocampal Alzheimer brain sections and are typical for this disease, but are rare or absent in healthy brain.
