MA5-24121

antibody from Invitrogen Antibodies

Targeting: LINGO2 LERN3, LRRN6C

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24121

Provider

Invitrogen Antibodies

Product name

LINGO2 Monoclonal Antibody (382007)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs and Western blots, no cross-reactivity with recombinant human LINGO-1 is observed.

Antibody clone number

382007

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LINGO2 in SH‚SY5Y human neuroblastoma cell line. Samples were incubated in LINGO2 monoclonal antibody (Product # MA5-24121) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for LINGO‚2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HEK293 human embryonic kidney cell line either (A) transfected with human LINGO-2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LINGO-2 Monoclonal Antibody (Product # MA5-24121) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody. Quadrant markers were set based on control antibody staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of LINGO2 in A549 human lung carcinoma cell line. Samples were incubated in LINGO2 monoclonal antibody (Product # MA5-24121) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of LINGO2 in HEK293 human embryonic kidney cell line either (A) transfected with human LINGO-2 or (B) irrelevant transfectants and eGFP. Samples were incubated in LINGO2 monoclonal antibody (Product # MA5-24121) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody. Quadrant markers were set based on control antibody staining.