PAB4256

antibody from Abnova Corporation

Targeting: ARL1 ARFL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB4256

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB4256, RRID:AB_1571357

Product name

ARL1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of ARL1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Phospholipid- and GTP-dependent activation of cholera toxin and phospholipase D by human ADP-ribosylation factor-like protein 1 (HARL1).

Hong JX, Lee FJ, Patton WA, Lin CY, Moss J, Vaughan M
The Journal of biological chemistry 1998 Jun 19;273(25):15872-6

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Different ARF domains are required for the activation of cholera toxin and phospholipase D.

Zhang GF, Patton WA, Lee FJ, Liyanage M, Han JS, Rhee SG, Moss J, Vaughan M
The Journal of biological chemistry 1995 Jan 6;270(1):21-4

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of K-562 cell lysate (35 ug/lane) with ARL1 polyclonal antibody (Cat # PAB4256).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ARL1 polyclonal antibody (Cat # PAB4256) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)