Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 12645-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GlnRS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: VKYTPHHRT GDKWCIYPTY DYTHCLCDSI EHITHSLCTK EFQARRSSYF WLCNALDVYC PVQWEYGRLN LHYAVVSKRK ILQLVATGAV RDWDDPRLFT LTALRRRGFP PEAINNFCAR VGVTVAQTTM EPHLLEACVR DVLNDTAPRA MAVLESLRVI ITNFPAAKSL DIQVPNFPAD ETKGFHQVPF APIVFIERTD FKEEPEPGFK RLAWGQPVGL RHTGYVIELQ HVVKGPSGCV ESLEVTCRRA DAGEKPKAFI HWVSQPLMCE VRLYERLFQH KNPEDPTEVP GGFLSDLNLA SLHVVDAALV DCSVALAKPF DKFQFERLGY FSVDPDSHQG KLVFNRTVTL KEDPGKV (362-717 aa encoded by BC016634 )
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PC-3 cells using 12645-1-AP (GlnRS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 12645-1-AP (GlnRS antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GlnRS (IP:12645-1-AP, 4ug; Detection:12645-1-AP 1:500) with PC-3 cells lysate 3200ug.