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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF5826 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SULT4A1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SULT1A1, rhSULT2A1, and rhSULT1E1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9BR01- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 by Western Blot. Western blot shows lysates of human brain cortex and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5826) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 4A1/SULT4A1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SULT4A1 in Human Brain SULT4A1 was detected in paraffin-embedded sections of human brain (cortex) using Goat Anti-Human SULT4A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5826) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
