MA5-24251

antibody from Invitrogen Antibodies

Targeting: CLC Gal-10, LGALS10, MGC149659

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24251

Provider

Invitrogen Antibodies

Product name

Galectin 10 Monoclonal Antibody (561603)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, no cross-reactivity with recombinant human Galectin-1, -2, -3, -4, -7, -8, or -9/Ecalectin is observed.

Antibody clone number

561603

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of human eosinophils (enriched, approximately 60%). PVDF Membrane was probed with 1 µg/mL of human Galectin-10 Monoclonal Antibody (Product # MA5-24251) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Galectin-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Galectin 10 in human eosinophils (enriched, approximately 60%). Samples were incubated in Galectin 10 monoclonal antibody (Product # MA5-24251) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Galectin‚10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Galectin 10 in immersion fixed HL‚60 human acute promyelocytic leukemia cell line. Samples were incubated in Galectin 10 monoclonal antibody (Product # MA5-24251) using a dilution of 8 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of human peripheral blood lymphocytes were stained with Human Galectin-10 Monoclonal Antibody (Product # MA5-24251) followed by FITC-conjugated Anti-Mouse IgG Secondary Antibody and Human IL-2Rα APC-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Galectin 10 in Human peripheral blood lymphocytes. Samples were incubated in Galectin 10 monoclonal antibody (Product # MA5-24251) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody and Human IL‚2 Rα APC-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining.