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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-97239 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP19 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MSGGASATGP RRGPPGLEDT TSKKKQKDRA NQESKDGDPR KETGSRYVAQ AGLEPLASGD PSASASHAAG ITGSRHRTRL FFPSSSGSAS TPQEEQTKEG ACEDPHDLLA TPTPELLLDW RQSAEEVIVK LRVGVGPLQL EDVDAAFTDT DCVVRFAGGQ QWGGVFYAEI KSSCAKVQTR KGSLLHLTLP KKVPMLTWPS; Positive Samples: U-87MG, A-431, HeLa; Cellular Location: Endoplasmic reticulum membrane, Single-pass membrane protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.10 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.
Culver JA, Mariappan M
The Journal of cell biology 2021 May 3;220(5)
The Journal of cell biology 2021 May 3;220(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of USP19 in extracts of various cell lines using USP19 Polyclonal Antibody (Product # PA5-97239) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of USP19 was performed in L929 cells using USP19 Polyclonal Antibody (Product # PA5-97239) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. ER-localized USP20 and USP33 are required for deubiquitination of TA proteins . (A) HEK293 cells with the indicated CRISPR/Cas9 knockout cell lines were transfected with VAPA-FLAG and HA-ubiquitin. VAPA was immunoprecipitated (IP) and immunoblotted (IB) with anti-FLAG antibody for VAPA and anti-HA antibody for ubiquitinated (Ub) VAPA. The orange circle indicates the glycosylated form of VAPA. (B) WT HEK293 and USP20/33 -/- cells were fractionated into cytosol (cyto.) and membrane (mem.) using 0.015% digitonin. Immunoblotting of TRAPalpha (membrane control) and SGTA (cytosolic control) shows the successful separation of the cytosol and membrane. Data are representative of two independent experiments. (C) Increasing amounts of rough microsomes isolated from HEK293 cells were immunoblotted for USP20, USP33, and the membrane protein Sec61alpha as a control. (D) WT, USP20 -/- , and USP33 -/- HEK293 cells were stained for the indicated endogenous proteins. Confocal imaging shows colocalization signal (yellow) of USP33 (red) and calreticulin (green). Scale bar, 10 um. Data are representative of two independent experiments. (E) HeLa cells were transfected with the ER control protein PDI and either USP20 or USP33 and then treated as in C for confocal microscopy. Scale bar, 10 um.
