25233-1-AP

antibody from Invitrogen Antibodies

Targeting: MARF1 KIAA0430, LKAP, LMKB, PPP1R34

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

25233-1-AP

Provider

Invitrogen Antibodies

Product name

KIAA0430 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MMEGNGTEN SCSRTRGWLQ QDNDAKPWLW KFSNCFSRPE QTLPHSPQTK EYMENKKVAV ELKDVPSPLH AGSKLFPAVP LPDIRSLQQP KIQLSSVPKV SCCAHCPNEP STSPMRFGGG GGGSGGTSSL IHPGALLDSQ STRTITCQVG SGFAFQSASS LQNASARNNL AGIASDFPSM CLESNLSSCK HLPCCGKLHF QSCHGNVHKL HQFPSLQGCT SAGYFPCSDF TSGAPGHLEE HISQSELTPH LCTNSLHLNV VPPVCLKGSL YCEDCLNKPA RNSIIDA (1-286 aa encoded by BC137165)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.31 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 25233-1-AP ( KIAA0430 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 25233-1-AP (KIAA0430 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 25233-1-AP (KIAA0430 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tissue slide using 25233-1-AP (KIAA0430 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 25233-1-AP (KIAA0430 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tissue slide using 25233-1-AP ( KIAA0430 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 25233-1-AP ( KIAA0430 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-KIAA0430 (IP:25233-1-AP, 5ug; Detection:25233-1-AP 1:300) with HeLa cells lysate 2000ug.