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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00004723-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004723-M01, RRID:AB_2266898
- Product name
- Mouse Monoclonal NDUFV1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. NDUFV1 (4A7)
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Human ind1, an iron-sulfur cluster assembly factor for respiratory complex I.
Sheftel AD, Stehling O, Pierik AJ, Netz DJ, Kerscher S, Elsässer HP, Wittig I, Balk J, Brandt U, Lill R
Molecular and cellular biology 2009 Nov;29(22):6059-73
Molecular and cellular biology 2009 Nov;29(22):6059-73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NDUFV1 Antibody (4A7) [H00004723-M01] - Analysis of NDUFV1 over-expressed 293 cell line, cotransfected with NDUFV1 Validated Chimera RNAi ( Cat # H00004723-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFV1 monoclonal antibody (M01), clone 4A7 (Cat # H00004723-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NDUFV1 Antibody (4A7) [H00004723-M01] - Analysis of NDUFV1 expression in transfected 293T cell line by NDUFV1 monoclonal antibody (M01), clone 4A7.Lane 1: NDUFV1 transfected lysate(50.8 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NDUFV1 Antibody (4A7) [H00004723-M01] - NDUFV1 monoclonal antibody (M01), clone 4A7. Analysis of NDUFV1 expression in PC-12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: NDUFV1 Antibody (4A7) [H00004723-M01] - Detection limit for recombinant GST tagged NDUFV1 is approximately 1ng/ml as a capture antibody.
