Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- GTX110037 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110037, RRID:AB_1950924
- Product name
- MCD antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 7.5% SDS PAGE GTX110037 diluted at 1:1000
- Validation comment
- WB
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- GeneTex (provider)
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- Experimental details
- Sample (30 ug of whole cell lysate)A: Hep G2 (GTX27900)7.5% SDS PAGEGTX110037 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
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- Experimental details
- MCD antibody detects MCD protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract7.5% SDS-PAGEMCD antibody (GTX110037) dilution: 1:500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCD antibody detects MCD protein by western blot analysis.A. 30 ?g C8D30 whole cell lysate/extract7.5% SDS-PAGEMCD antibody (GTX110037) dilution: 1:500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCD(GTX110037) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H661 xenograft, using MCD(GTX110037) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MCD antibody [N2C1], Internal (GTX110037) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. MCD antibody [N2C1], Internal (GTX110037) dilution: 1:500.