24284-1-AP

antibody from Invitrogen Antibodies

Targeting: PAPOLG FLJ12972

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

24284-1-AP

Provider

Invitrogen Antibodies

Product name

PAPOLG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: QANNINMLK EGMKIEATHV KKKQLHHYLP AEILQKKKKQ SLSDVNRSSG GLQSKRLSLD SSCLDSSRDT DNGTPFNSPA SKSDSPSVGE TERNSAEPAA VIVEKPLSVP PAQGLSIPVI GAKVDSTVKT VSPPTVCTIP TVVGRNVIPR ITTPHNPAQG QPHLNGMSNI TKTVTPKRSH SPSIDGTPKR LKDVEKFIRL ESTFKDPRTA EERKRKSVDA IGGESMPIPT IDTSRKKRLP SKELPDSSSP VPANNIRVIK NSIRLTLNR (469-736 aa encoded by BC111701)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 24284-1-AP ( PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 24284-1-AP (PAPOLG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 24284-1-AP ( PAPOLG Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PAPOLG (IP:24284-1-AP, 4ug; Detection:24284-1-AP 1:1000) with HeLa cells lysate 3200ug.