TA504546

antibody from OriGene

Targeting: DNAAF1 CILD13, DAU1, FLJ25330, LRRC50, ODA7, swt

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504546

Provider

OriGene

Proper citation

OriGene Cat#TA504546, RRID:AB_2622465

Product name

LRRC50 mouse monoclonal antibody, clone OTI2C4 (formerly 2C4)

Antibody type

Monoclonal

Description

LRRC50 mouse monoclonal antibody, clone OTI2C4 (formerly 2C4)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

DNAAF1

Isotype

IgG

Antibody clone number

OTI2C4

Vial size

100 µl

Concentration

2.74 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC205452 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LRRC50 antibody(TA504546), and then analyzed by flow cytometry.

Validation comment

FC