HPA003998

antibody from Atlas Antibodies

Targeting: MAGEA8 CT1.8, MAGE8, MGC2182

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA003998

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003998, RRID:AB_10965020

Product name

Anti-MAGEA8

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AQGEAPGLMDVQIPTAEEQKAASSSSTLIMGTLEE
VTDSGSPSPPQSPEGASSSLTVTDSTLWSQSDEGS
SSNEEEGPSTSPDPAHLESLFREALDEKVAELVRF
LLRKYQIKEPVTKAEMLESVI


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human placenta and prostate tissues using Anti-MAGEA8 antibody. Corresponding MAGEA8 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts and strong cytoplasmic positivity in Leydig cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows low expression as expected.

Sample type

HUMAN