14-1009-37

antibody from Invitrogen Antibodies

Targeting: SEMA4D C9orf164, CD100, coll-4, FLJ39737, SEMAJ

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

14-1009-37

Provider

Invitrogen Antibodies

Product name

CD100 (SEMA4D) Monoclonal Antibody (eBio133-1C6 (133-1C6)), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The eBio133-1C6 monoclonal antibody reacts with human CD100, a member of the class IV semaphorin family also called sema4D. CD100 promotes angiogenesis, invasive growth, proliferation, and anti-apoptosis of cancer cells in vitro. Furthermore CD100 is a costimulatory molecule in adaptive immune responses by switching off negative signalling from CD72. In addition to CD72 another ligand is Plexin-B1. It has been shown that higher expression levels of CD100 are correlated with poor survival in soft tissue sarcoma patients. Expression is found in both hematopoietic and non-hematopoietic cells.CD100 is expressed on resting T cells and NK cells and B cell. CD100 can also exist as a soluble form.

Antibody clone number

eBio133-1C6 (133-1C6)

Concentration

0.5 mg/mL

References

Submitted references

Soluble Sema4D in Plasma of Head and Neck Squamous Cell Carcinoma Patients Is Associated With Underlying Non-Inflamed Tumor Profile.

Younis RH, Ghita I, Elnaggar M, Chaisuparat R, Theofilou VI, Dyalram D, Ord RA, Davila E, Tallon LJ, Papadimitriou JC, Webb TJ, Bentzen SM, Lubek JE
Frontiers in immunology 2021;12:596646

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgM Isotype Control Purified, (Product # 14-4752-82) (open histogram) or Anti-Human CD100 (SEMA4D) Purified (filled histogram) followed by Anti-Mouse IgM PE (Product # 12-5890-82). Cells in the lymphocyte gate were used for analysis.