328401

antibody from BioLegend

Targeting: SEMA4D C9orf164, CD100, coll-4, FLJ39737, SEMAJ

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

328401

Provider

BioLegend

Proper citation

BioLegend Cat#328401, RRID:AB_1236386

Product name

Purified anti-human CD100

Antibody type

Monoclonal

Antigen

Human PHA activated lymphocytes

Description

CD100, a 150 kD homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Reactivity

Human, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes stained with purified A8, followed by anti-mouse IgG FITC

Conjugate

Unconjugated