HPA015662

antibody from Atlas Antibodies

Targeting: SEMA4D C9orf164, CD100, coll-4, FLJ39737, SEMAJ

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA015662

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA015662, RRID:AB_1856671

Product name

Anti-SEMA4D

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LLIGKKKPKSDFCDREQSLKETLVEPGSFSQQNGE
HPKPALDTGYETEQDTITSKVPTDREDSQRIDDLS
ARDKPFDVKCELKFADSDADGD


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and SEMA4D over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416686).

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line HDLM-2.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows cytoplasmic positivity in germinal center cells and non-germinal center cells.

Sample type

HUMAN