Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-99691 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR3B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis.
Chen Q, Ma K, Liu X, Chen SH, Li P, Yu Y, Leung AKL, Yu X
Cell discovery 2022 Jan 18;8(1):3
Cell discovery 2022 Jan 18;8(1):3
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR3B in LOVO cell lysate. Samples were incubated with POLR3B polyclonal antibody (Product # PA5-99691).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of POLR3B in LOVO. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with POLR3B polyclonal antibody (Product # PA5-99691) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 The BRCT domain of tPARP1 interacts with the Pol III complex. a U2OS-PARP1 knockout cells were transfected with the myc-empty vector (EV), myc-POLR3A, myc-POLR3B, or myc-POLR3F together with HA-mtPARP1, followed by IP and western blotting with the indicated antibodies. b mtPARP1 or full-length mPARP1 was ectopically expressed in U2OS-PARP1 knockout cells. IP was performed with IgG as a negative control. c U2OS-PARP1 knockout cells were transfected with the myc-empty vector (EV), myc-POLR3A, myc-POLR3B, or myc-POLR3F together with HA-mTARG1, followed by IP and western blotting with the indicated antibodies. d U2OS-PARP1 knockout cells were transfected with the HA-tagged truncation mutants of mtPARP1 together with myc-POLR3A (upper panel), myc-POLR3B (middle panel), or myc-POLR3F (lower panel). e U2OS-PARP1 knockout cells were transfected with the indicated combinations of HA-tagged mtPARP1 or mtPARP1 F473A mutant together with myc-POLR3A (upper panel), myc-POLR3B (middle panel), or myc-POLR3F (lower panel).