ALX-803-080-C100

antibody from Enzo Life Sciences

Targeting: MAP1LC3B ATG8F

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ALX-803-080-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-803-080-C100, RRID:AB_2051773

Product name

LC3B monoclonal antibody (5F10)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography.

Reactivity

Human, Mouse, Rat, Canine, Hamster

Host

Mouse

Isotype

IgG

Antibody clone number

5F10

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.

References

Submitted references

The nuclear cofactor DOR regulates autophagy in mammalian and Drosophila cells.

Mauvezin C, Orpinell M, Francis VA, Mansilla F, Duran J, Ribas V, Palacín M, Boya P, Teleman AA, Zorzano A
EMBO reports 2010 Jan;11(1):37-44

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Discovery of Atg5/Atg7-independent alternative macroautophagy.

Nishida Y, Arakawa S, Fujitani K, Yamaguchi H, Mizuta T, Kanaseki T, Komatsu M, Otsu K, Tsujimoto Y, Shimizu S
Nature 2009 Oct 1;461(7264):654-8

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Early endosomes and endosomal coatomer are required for autophagy.

Razi M, Chan EY, Tooze SA
The Journal of cell biology 2009 Apr 20;185(2):305-21

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Endogenous LC3 punctae detected using MAb to LC3B (5F10) (Prod. No. ALX-803-080)Method: The majority of LC3 was diffusely localized in unstimulated COS-7 cells, wheres punctated signals of LC3 increase after induction of autophagy by vinblastin stimulation for 2h. Cells were fixed with paraformaldehyde followed by methanol treatment. Cells were permeabilized with 0.3% Triton X-100. (Image courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK.)